seqWell Launches Loaded-transposase Portfolio to Support Expanding Use of NGS-based Gene Editing Analysis Methods
2024年6月26日 - 11:05PM
ビジネスワイヤ(英語)
Tagify™ off-the-shelf and custom
loaded-transposase reagents provide reliability and scalability to
tagmentation-based on- & off-target gene editing analysis
seqWell, a global provider of genomic library and multiplexing
workflow solutions, today announced the launch of their new
portfolio of Tagify loaded-transposase reagents, including Tagify
i5 UMI adapter-loaded Tn5. In addition, seqWell will offer loading
of transposases with customer-specified oligonucleotides, including
loading of their high-performance TnX transposase.
This press release features multimedia. View
the full release here:
https://www.businesswire.com/news/home/20240626702033/en/
Tagify Adapter-loaded Transposase
Reagents provide for reliable and scalable NGS-based on- and
off-target gene editing analysis methods. (Photo: Business
Wire)
The Tagify portfolio provides a commercial source of fully
quality-controlled, loaded transposases with a defined level of
enzyme activity and payload verification. This eliminates the need
of laboratories to purify, load, and characterize the final loaded
transposase prior to use in their assays, freeing up laboratory
resources and reducing a source of assay-to-assay variability.
“Assays to define the genome-wide on- and off-target activity of
genome editors are important for both biomedical research and
therapeutics,” said Shengdar Tsai, Ph.D, Associate Member,
Department of Hematology, St. Jude Children’s Research Hospital.
“Broadly adopted, sensitive, unbiased, biochemical and cellular
methods of understanding the genome-wide activity of editors, such
as CHANGE-seq and GUIDE-seq-2, rely on transposase-based tools. The
commercial availability of standardized transposase reagents will
help lower barriers for more labs to implement these assays rapidly
and reliably.”
Tagify i5 UMI adapter-loaded transposase reagents support gene
editing analysis assays, such as the UdiTaS™ method, that rely on
tagmentation to simultaneously fragment and tag genomic DNA with
Illumina-compatible adapters using a highly scalable workflow.
These off-the-shelf reagents are available as a set of either 24 or
96 transposases each containing an i5 barcode and a unique
molecular indentifier (UMI) for high throughput applications.
The Tagify custom-loading option enables scientists to address
the growing number of NGS-based on- and off-target gene editing
methods that rely on tagmentation via the flexibility to load
either hyperactive Tn5 or seqWell’s recently introduced high
performance TnX transposase, with Illumina-compatible barcodes,
UMIs, universal adaptors, or a custom oligonucleotide sequence.
“Our early access customers valued the availability of a
reliable source of quality transposase-based reagents with their
payload of choice, and Tagify products build on seqWell’s expertise
in transposase loading and manufacturing to deliver these tools”
said Joe Mellor, Chief Scientific Officer at seqWell. “Tagify i5
UMI is a great example of this capability, focused on scientists
developing new genome editing methods and therapeutics. The high
level of batch-to-batch consistency and availability of up to 96
UMIs will support the reproducible and scalable analysis of on- and
off-target effects in sequencing-based genome editing QC
assays.”
For more information about the use of Tagify loaded transposase
reagents, view an on-demand webinar entitled, “Assessing CRISPR
On-Target Editing and Structural Changes with UDiTaS™ using Tagify™
Reagents” presented by Georgia Giannoukos, Ph.D., Director of Next
Generation Sequencing, at Editas Medicine.
About seqWell
seqWell creates scalable genomics technologies that simplify
workflows and help enable every life scientist to unlock
transformative discoveries with sequencing. seqWell offers a range
of next-generation library prep products and workflows that allow
customers to achieve higher levels of sample multiplexing quickly
and easily. Learn more about us at www.seqwell.com or follow us on
Linkedin.
View source
version on businesswire.com: https://www.businesswire.com/news/home/20240626702033/en/
Media Contact: Krista Steger seqWell, Inc.
krista.steger@seqwell.com